English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

High levels of salinity induce serious oxidative damage in plants. Flavonoids, as antioxidants, have important roles in reactive oxygen species (ROS) scavenging. In the present study, the tobacco R2R3 MYB type repressor, NtMYB4, was isolated and characterized. The expression of  NtMYB4  was suppressed by salinity. Overexpression of  NtMYB4  reduced the salt tolerance in transgenic tobacco plants. NtMYB4 repressed the promoter activity of  NtCHS1  and negatively regulated its expression. Rutin accumulation was significantly decreased in  NtMYB4  overexpressing transgenic plants and  NtCHS1  RNAi silenced transgenic plants. Moreover, high H 2 O 2  andO  2  −contents were detected in both types of rutin-reduced transgenic plants under high salt stress. In addition, exogenous rutin supplementation effectively scavenged ROS (H 2 O 2  andO  2  −) and improved the salt tolerance of the rutin-reduced transgenic plants. In contrast,  NtCHS1  overexpressing plants had increased rutin accumulation, lower H 2 O 2  andO  2  −contents, and higher tolerance to salinity. These results suggested that tobacco NtMYB4 acts as a salinity response repressor and negatively regulates  NtCHS1  expression, which results in the reduced flavonoid accumulation and weakened ROS-scavenging ability under salt stress.

NtMYB4 and NtCHS1 Are Critical Factors in the Regulation of Flavonoid Biosynthesis and Are Involved in Salinity Responsiveness