English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Guayule ( Parthenium argentatum ) is a perennial shrub in the Asteraceae family and synthesizes a high quality, hypoallergenic  cis -1,4-polyisoprene (or natural rubber; NR). Despite its potential to be an alternative NR supplier, the enzymes for  cis -polyisoprene biosynthesis have not been comprehensively studied in guayule. Recently, implications of the protein complex involving  cis -prenyltransferases (CPTs) and CPT-Binding Proteins (CBPs) in NR biosynthesis were shown in lettuce and dandelion, but such protein complexes have yet to be examined in guayule. Here, we identified four guayule genes – three  PaCPTs  ( PaCPT1-3 ) and one  PaCBP , whose protein products organize PaCPT/PaCBP complexes. Co-expression of both  PaCBP  and each of the  PaCPTs  could complemented the dolichol (a short  cis -polyisoprene)-deficient yeast, whereas the individual expressions could not. Microsomes from the  PaCPT/PaCBP -expressing yeast efficiently incorporated  14 C-isopentenyl diphosphate into dehydrodolichyl diphosphates; however, NR with high molecular weight could not be synthesized in  in vitro  assays. Furthermore, co-immunoprecipitation and split-ubiquitin yeast 2-hybrid assays using PaCPTs and PaCBP confirmed the formation of protein complexes. Of the three  PaCPT s, guayule transcriptomics analysis indicated that the  PaCPT3  is predominantly expressed in stem and induced by cold-stress, suggesting its involvement in NR biosynthesis. The comprehensive analyses of these PaCPTs and PaCBP here provide the foundational knowledge to generate a high NR-yielding guayule.

Molecular Studies of the Protein Complexes Involving Cis-Prenyltransferase in Guayule (Parthenium argentatum), an Alternative Rubber-Producing Plant