English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Functional conservation of RNAs between different species is a key argument for their importance. While few long non-coding RNAs are conserved at the sequence level, many long non-coding RNAs have been identified that only share a position relative to other genes. It remains largely unknown whether and how these lncRNAs are conserved beyond their position. In  Arabidopsis thaliana , the lncRNA  COOLAIR  is transcribed antisense from  FLOWERING LOCUS C  ( FLC ) in response to cold. Despite relatively low sequence similarity, the  COOLAIR  expression pattern and  in vitro  RNA secondary structure are highly conserved across the family Brassicaceae, which originated some 50 mya. It is unclear, however, whether  COOLAIR  functions in distantly related species such as monocots, which diverged some 150 mya. Here, we identified antisense lncRNAs from  FLC  homologs in various monocot species that share no sequence similarity with  A. thaliana COOLAIR . Yet similar to  COOLAIR , we found that  BdODDSOC1  antisense ( BdCOOLAIR1 ) and  BdODDSOC2  antisense ( BdCOOLAIR2 ) are induced by cold in a  Brachypodium distachyon  winter accession. Across  B. distachyon  accessions, the sequences of  BdCOOLAIR1  and  BdCOOLAIR2  are less conserved than exons but more conserved than flanking regions, suggesting a function for the transcript itself. Knock down of the  BdODDSOC2  non-overlapping  BdCOOLAIR2  transcript did not show a morphological phenotype, but did result in significantly higher  BdODDSOC2  expression during cold, indicating that  BdCOOLAIR2  performs a role  in cis  in the rate of  BdODDSOC2  silencing. This functional similarity between eudicot and monocot species reveals ancient conservation or convergent evolution of  FLC  antisense transcription. Either scenario supports its functional importance.

Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses