English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Potassium (K + ) is the most abundant cation in plants, and its uptake and transport are key to growth, development and responses to the environment. Here, we report that  Arabidopsis thaliana  K +  uptake permease 5 (AtKUP5) contains an adenylate cyclase (AC) catalytic center embedded in its N-terminal cytosolic domain. The purified recombinant AC domain generates cAMP  in vitro ; and when expressed in  Escherichia coli , increases cAMP levels  in vivo . Both the AC domain and full length AtKUP5 rescue an AC-deficient  E. coli  mutant,  cyaA , and together these data provide evidence that AtKUP5 functions as an AC. Furthermore, full length AtKUP5 complements the  Saccharomyces cerevisiae  K +  transport impaired mutant,  trk1 trk2 , demonstrating its function as a K +  transporter. Surprisingly, a point mutation in the AC center that impairs AC activity, also abolishes complementation of  trk1 trk2 , suggesting that a functional catalytic AC domain is essential for K +  uptake. AtKUP5-mediated K +  uptake is not affected by cAMP, the catalytic product of the AC, but, interestingly, causes cytosolic cAMP accumulation. These findings are consistent with a role for AtKUP5 as K +  flux sensor, where the flux-dependent cAMP increases modulate downstream components essential for K +  homeostasis, such as cyclic nucleotide gated channels.

The Arabidopsis thaliana K+-Uptake Permease 5 (AtKUP5) Contains a Functional Cytosolic Adenylate Cyclase Essential for K+ Transport