English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Nucleobase:cation symporter 2 (NCS2) proteins are important for the transport of free nucleobases, participating in diverse plant growth and developmental processes, as well as response to abiotic stress. To date, a comprehensive analysis of the  NCS2  gene family has not been performed in maize. In this study, we conducted a comparative genomics analysis of  NCS2  genes in 28 plant species, ranging from aquatic algae to land plants, concentrating mainly on maize. Gene duplication events contributed to the expansion of  NCS2  genes from lower aquatic plants to higher angiosperms, and whole-genome/segmental and single-gene duplication events were responsible for the expansion of the maize  NCS2  gene family. Phylogenetic construction showed three  NCS2  subfamilies, I, II, and III. According to homology-based relationships, members of subfamily I are  NCS2/AzgA-like  genes, whereas those in subfamilies II and III are  NCS2/NAT s. Moreover, subfamily I exhibited ancient origins. A motif compositional analysis showed that one symbolic motif (motif 4) of the  NCS2/NAT  genes was absent in subfamily I. In maize, three  NCS2/AzgA-like  and 21  NCS2/NAT  genes were identified, and purifying selection influenced the duplication of maize  NCS2  genes. Additionally, a population genetic analysis of  NCS2  genes revealed that  ZmNCS2–21  showed the greatest diversity between the 78 inbred and 22 wild surveyed maize populations. An expression profile analysis using transcriptome data and quantitative real-time PCR revealed that  NCS2  genes in maize are involved in diverse developmental processes and responses to abiotic stresses, including abscisic acid, salt (NaCl), polyethylene glycol, and low (4°C) and high (42°C) temperatures.  ZmNCS2  genes with relatively close relationships had similar expression patterns, strongly indicating functional redundancy. Finally,  ZmNCS2–16  and  ZmNCS2–23  localize in the plasma membrane, which confirmed their predicted membrane structures. These results provide a foundation for future studies regarding the functions of ZmNCS2 proteins, particularly those with potentially important roles in plant responses to abiotic stresses.

Comparative Genomics, Whole-Genome Re-sequencing and Expression Profile Analysis of Nucleobase:Cation Symporter 2 (NCS2) Genes in Maize