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Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of  Erigeron breviscapus  (Vaniot) Hand.-Mazz. used for the treatment of cerebrovascular diseases. However, due to overexploitation and overuse,  E. breviscapus  is facing the problems of extinction and habitat degradation. In this study, a correlation analysis between the transcript and metabolite profiles of methyl jasmonate (MeJA)-treated  E. breviscapus  at different time points indicated that chalcone isomerase ( Eb CHI) was the primary contributor to scutellarin accumulation during flavonoid biosynthesis.  Eb CHI was then further characterized as a chalcone isomerase that efficiently converted chalcone to naringenin  in vitro . Optimal parameters derived by comparing different culture conditions were successfully used to establish hairy root cultures of  E. breviscapus  with a maximum transformation rate of 60% in B5 medium. Furthermore, overexpression of  Eb CHI significantly enhanced scutellarin accumulation in  E. breviscapus  hairy roots with a maximum content of 2.21 mg g -1  (dw), 10-fold higher than that of natural roots (0.21 mg g -1  dw). This study sheds new light on a method of effective gene-based metabolic engineering by accurate and appropriate strategies and provides a protocol for hairy root cultures that accumulate high levels of scutellarin, providing a promising prospect for relieving the overexploitation and unavailability of  E. breviscapus  in the future.

frontiers in plant science

Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots