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Bioaccumulation of arsenic (As) in rice ( Oryza sativa ) increases human exposure to this toxic, carcinogenic element. Recent studies identified several As transporters, but the regulation of these transporters remains unclear. Here, we show that the rice R2R3 MYB transcription factor OsARM1 ( A RSENITE- R ESPONSIVE  M YB 1 ) regulates As-associated transporters genes. Treatment with As(III) induced  OsARM1  transcript accumulation and an OsARM1-GFP fusion localized to the nucleus. Histochemical analysis of  OsARM1pro::GUS  lines indicated that  OsARM1  was expressed in the phloem of vascular bundles in basal and upper nodes. Knockout of  OsARM1  ( OsARM1-KO  CRISPR/Cas9-generated mutants) improved tolerance to As(III) and overexpression of  OsARM1  ( OsARM1-OE  lines) increased sensitivity to As(III). Measurement of As in As(III)-treated plants showed that under low As(III) conditions (2 μM), more As was transported from the roots to the shoots in  OsARM1-KOs . By contrast, more As accumulated in the roots in  OsARM1-OEs  in response to high As(III) exposure (25 μM). In particular, the As(III) levels in node I were significantly higher in  OsARM1-KOs , but significantly lower in  OsARM1-OEs , compared to wild-type plants, implying that OsARM1 is important for the regulation of root-to-shoot translocation of As. Moreover,  OsLsi1, OsLsi2 , and  OsLsi6 , which encode key As transporters, were significantly downregulated in  OsARM1-OEs  and upregulated in  OsARM1-KOs  compared to wild type. Chromatin immunoprecipitation-quantitative PCR of  OsARM1-OEs  indicated that OsARM1 binds to the conserved MYB-binding sites in the promoters or genomic regions of  OsLsi1, OsLsi2 , and  OsLsi6  in rice. Our findings suggest that the OsARM1 transcription factor has essential functions in regulating As uptake and root-to-shoot translocation in rice.

OsARM1, an R2R3 MYB Transcription Factor, Is Involved in Regulation of the Response to Arsenic Stress in Rice