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Although adenosine monophosphate (AMP) binding domain is widely distributed in multiple plant species, detailed molecular functions of AMP binding proteins (AMPBPs) in plant development and plant-pathogen interaction remain unclear. In the present study, we identified an AMPBP  OsAAE3  from a previous analysis of early responsive genes in rice during  Magnaporthe oryzae  infection.  OsAAE3  is a homolog of Arabidopsis  AAE3  in rice, which encodes a 4-coumarate-Co-A ligase (4CL) like protein. A phylogenetic analysis showed that  OsAAE3  was most likely  4CL-like 10  in an independent group.  OsAAE3  was localized to cytoplasm, and it could be expressed in various tissues. Histochemical staining of transgenic plants carrying  OsAAE3  promoter-driven GUS (β-glucuronidase) reporter gene suggested that  OsAAE3  was expressed in all tissues of rice. Furthermore,  OsAAE3-OX  plants showed increased susceptibility to  M. Oryzae , and this finding was attributable to decreased expression of pathogen-related 1a ( PR1 ) and low level of peroxidase (POD) activity. Moreover,  OsAAE3  over-expression resulted in increased content of H 2 O 2 , leading to programmed cell-death induced by reactive oxygen species (ROS). In addition,  OsAAE3  over-expression repressed the floret development, exhibiting dramatically twisted glume and decreased fertility rate of anther. Meanwhile, the expressions of lignin biosynthesis genes were significantly decreased in  OsAAE3-OX  plants, thereby leading to reduced lignin content. Taken together,  OsAAE3  functioned as a negative regulator in rice blast resistance, floret development, and lignin biosynthesis. Our findings further expanded the knowledge in functions of AMBPs in plant floret development and the regulation of rice-fungus interaction.

4-Coumarate-CoA Ligase-Like Gene OsAAE3 Negatively Mediates the Rice Blast Resistance, Floret Development and Lignin Biosynthesis