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Senescence is not only an important developmental process, but also a responsive regulation to abiotic and biotic stress for plants. Stay-green protein plays crucial roles in plant senescence and chlorophyll degradation. However, the underlying mechanisms were not well-studied, particularly in non-model plants. In this study, a novel stay-green gene,  ZjSGR , was isolated from  Zoysia japonica . Subcellular localization result demonstrated that ZjSGR was localized in the chloroplasts. Quantitative real-time PCR results together with promoter activity determination using transgenic Arabidopsis confirmed that  ZjSGR  could be induced by darkness, ABA and MeJA. Its expression levels could also be up-regulated by natural senescence, but suppressed by SA treatments. Overexpression of  ZjSGR  in Arabidopsis resulted in a rapid yellowing phenotype; complementary experiments proved that  ZjSGR  was a functional homolog of  AtNYE1  from  Arabidopsis thaliana . Over expression of  ZjSGR  accelerated chlorophyll degradation and impaired photosynthesis in Arabidopsis. Transmission electron microscopy observation revealed that overexpression of  ZjSGR  decomposed the chloroplasts structure. RNA sequencing analysis showed that  ZjSGR  could play multiple roles in senescence and chlorophyll degradation by regulating hormone signal transduction and the expression of a large number of senescence and environmental stress related genes. Our study provides a better understanding of the roles of  SGR s, and new insight into the senescence and chlorophyll degradation mechanisms in plants.

frontiers in plant science

Functional and RNA-Sequencing Analysis Revealed Expression of a Novel Stay-Green Gene from Zoysia japonica (ZjSGR) Caused Chlorophyll Degradation and Accelerated Senescence in Arabidopsis