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In higher plants the  L -galactose pathway represents the major route for ascorbate biosynthesis. The first committed step of this pathway is catalyzed by the enzyme GDP- L -galactose phosphorylase and is encoded by two paralogs in  Arabidopsis  –  VITAMIN C2  ( VTC2 ) and  VTC5 . The first mutant of this enzyme,  vtc2-1 , isolated via an EMS mutagenesis screen, has approximately 20–30% of wildtype ascorbate levels and has been reported to have decreased growth under standard laboratory conditions. Here, we show that a T-DNA insertion into the  VTC2  causes a similar reduction in ascorbate levels, but does not greatly affect plant growth. Subsequent segregation analysis revealed the growth defects of  vtc2-1  mutants segregate independently of the  vtc2-1  mutation. These observations suggest that it is the presence of an independent cryptic mutation that affects growth of  vtc2-1  mutants, and not the 70–80% decrease in ascorbate levels that has been assumed in past studies.

Ascorbate-Deficient vtc2 Mutants in Arabidopsis Do Not Exhibit Decreased Growth