English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Thidiazuron ( N -phenyl- N ′-1,2,3-thiadiazol-5-ylurea; TDZ) is an artificial plant growth regulator that is widely used in plant tissue culture. Protocorm-like bodies (PLBs) induced by TDZ serve as an efficient and rapid  in vitro  regeneration system in  Rosa  species. Despite this, the mechanism of PLB induction remains relatively unclear. TDZ, which can affect the level of endogenous auxins and cytokinins, converts the cell fate of rhizoid tips and triggers PLB formation and plantlet regeneration in  Rosa canina  L. In callus-rhizoids, which are rhizoids that co-develop from callus, auxin and a Z-type cytokinin accumulated after applying TDZ, and transcription of the auxin transporter gene  RcPIN1  was repressed. The expression of  RcARF4 ,  RcRR1 ,  RcCKX2 ,  RcCKX3 , and  RcLOG1  increased in callus-rhizoids and rhizoid tips while the transcription of an auxin response factor ( RcARF1 ) and auxin transport proteins ( RcPIN2 ,  RcPIN3 ) decreased in callus-rhizoids but increased in rhizoid tips.  In situ  hybridization of rhizoids showed that  RcWUS  and  RcSERK1  were highly expressed in columella cells and root stem cells resulting in the conversion of cell fate into shoot apical meristems or embryogenic callus. In addition, transgenic  XVE::RcWUS  lines showed repressed  RcWUS  overexpression while  RcWUS  had no effect on PLB morphogenesis. Furthermore, higher expression of the root stem cell marker  RcWOX5  and root stem cell maintenance regulator genes  RcPLT1  and  RcPLT2  indicated the presence of a dedifferentiation developmental pathway in the stem cell niche of rhizoids. Viewed together, our results indicate that different cells in rhizoid tips acquired regeneration competence after induction by TDZ. A novel developmental pathway containing different cell types during PLB formation was identified by analyzing the endogenous auxin and cytokinin content. This study also provides a deeper understanding of the mechanisms underlying  in vitro  regeneration in  Rosa .

Thidiazuron Triggers Morphogenesis in Rosa canina L. Protocorm-Like Bodies by Changing Incipient Cell Fate