English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Transformed hairy roots had been efficiently induced from the seedlings of  Fagopyrum tataricum  Gaertn. due to the infection of  Agrobacterium rhizogenes . Hairy roots were able to display active elongation with high root branching in 1/2 MS medium without growth regulators. The stable introduction of  rol B and  aux 1 genes of  A. rhizogenes  WT strain 15834 into  F. tataricum  plants was confirmed by PCR analysis. Besides, the absence of  vir D gene confirmed hairy root was bacteria-free. After six different media and different sources of concentration were tested, the culturing of TB7 hairy root line in 1/2 MS liquid medium supplemented with 30 g l -1  sucrose for 20 days resulted in a maximal biomass accumulation (13.5 g l -1  fresh weight, 1.78 g l -1  dry weight) and rutin content (0.85 mg g -1 ). The suspension culture of hairy roots led to a 45-fold biomass increase and a 4.11-fold rutin content increase in comparison with the suspension culture of non-transformed roots. The transformation frequency was enhanced through preculturing for 2 days followed by infection for 20 min. The UV-B stress treatment of hairy roots resulted in a striking increase of rutin and quercetin production. Furthermore, the hairy root lines of TB3, TB7, and TB28 were chosen to study the specific effects of UV-B on flavonoid accumulation and flavonoid biosynthetic gene expression by qRT-PCR. This study has demonstrated that the UV-B radiation was an effective elicitor that dramatically changed in the transcript abundance of  ftpAL ,  FtCHI ,  FtCHS ,  FtF3H , and  FtFLS-1  in  F. tataricum  hairy roots.

frontiers in plant science

Efficient Rutin and Quercetin Biosynthesis through Flavonoids-Related Gene Expression in Fagopyrum tataricum Gaertn. Hairy Root Cultures with UV-B Irradiation