English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Simple sugars, like glucose (Glc) and sucrose (Suc), act as signals to modulate the expression of hundreds of genes in plants. Frequently, however, it remains unclear whether this regulation is induced by the sugars themselves or by their derivatives generated in the course of carbohydrate (CH) metabolism. In the present study, we tested the relevance of different CH metabolism and allocation pathways affecting expression patterns of five selected sugar-responsive genes ( bZIP63, At5g22920, BT2, MGD2 , and  TPS9 ) in  Arabidopsis thaliana . In general, the expression followed diurnal changes in the overall sugar availability. However, under steady growth conditions, this response was hardly impaired in the mutants for CH metabolizing/ transporting proteins (a dg1, sex1, sus1-4, sus5/6 , and  tpt2 ), including also hexokinase1 (HXK1) loss- and gain-of-function plants— gin2.1  and  oe3.2 , respectively. In addition, transgenic plants carrying  pbZIP63::GUS  showed no changes in reporter-gene-expression when grown on sugar under steady-state conditions. In contrast, short-term treatments of agar-grown seedlings with 1% Glc or Suc induced  pbZIP63::GUS  repression, which became even more apparent in seedlings grown in liquid media. Subsequent analyses of liquid-grown  gin2.1  and  oe3.2  seedlings revealed that Glc -dependent regulation of the five selected genes was not affected in  gin2.1 , whereas it was enhanced in  oe3.2  plants for  bZIP63, At5g22920 , and  BT2 . The sugar treatments had no effect on ATP/ADP ratio, suggesting that changes in gene expression were not linked to cellular energy status. Overall, the data suggest that HXK1 does not act as Glc sensor controlling  bZIP63, At5g22920 , and  BT2  expression, but it is nevertheless required for the production of a downstream metabolic signal regulating their expression.

Hexokinase 1 is required for glucose-induced repression of bZIP63, At5g22920, and BT2 in Arabidopsis