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Murine slowly adapting receptors (SARs) within airway smooth muscle provide volume-related feedback; however, their mechanosensitivity and morphology are incompletely characterized. We explored two aspects of SAR physiology: their inherent static mechanosensitivity and a potential link to pulmonary neuroepithelial bodies (NEBs). SAR mechanosensitivity displays a rate sensitivity linked to speed of inflation; however, to what extent static SAR mechanosensitivity is tuned for the very rapid breathing frequency (B  f  ) of small mammals (e.g., mouse) is unclear. NEB-associated, morphologically described smooth muscle-associated receptors (SMARs) may be a structural analog for functionally characterized SARs, suggesting functional linkages between SARs and NEBs. We addressed the hypotheses that: (1) rapid murine B  f   is associated with enhanced  in vivo  SAR static sensitivity; (2) if SARs and NEBs are functionally linked, stimuli reported to impact NEB function would alter SAR mechanosensitivity. We measured SAR action potential discharge frequency (AP  f , action potentials/s) during quasi-static inflation [0–20 cmH 2 O trans-respiratory pressure (P TR )] in NEB-relevant conditions of hypoxia (F I O 2  = 0.1), hypercarbia (F I CO 2  = 0.1), and pharmacologic intervention (serotonergic 5-HT 3  receptor antagonist, Tropisetron, 4.5 mg/kg; P2 purinergic receptor antagonist, Suramin, 50 mg/kg). In all protocols, we obtained: (1) AP  f  vs. P TR ; (2) P TR  threshold; and (3) AP  f  onset at P TR  threshold. The murine AP  f  vs. P TR  response comprises high AP  f  (average maximum AP  f : 236.1 ± 11.1 AP/s at 20 cmH 2 O), a low P TR  threshold (mean 2.0 ± 0.1 cmH 2 O), and a plateau in AP  f  between 15 and 20 cmH 2 O. Murine SAR mechanosensitivity (AP  f  vs. P TR ) is up to 60% greater than that reported for larger mammals. Even the maximum difference between intervention and control conditions was minimally impacted by NEB-related alterations: Tropisetron −7.6 ± 1.8% ( p  = 0.005); Suramin −10.6 ± 1.5% ( p  = 0.01); hypoxia +9.3 ± 1.9% ( p  &amp;lt; 0.001); and hypercarbia −6.2 ± 0.9% ( p  &amp;lt; 0.001). We conclude that the high sensitivity of murine SARs to inflation provides enhanced resolution of operating lung volume, which is aligned with the rapid B  f   of the mouse. We found minimal evidence supporting a functional link between SARs and NEBs and speculate that the &amp;lt;10% change in SAR mechanosensitivity during altered NEB-related stimuli is not consistent with a meaningful physiologic role.

Mechanosensitivity of Murine Lung Slowly Adapting Receptors: Minimal Impact of Chemosensory, Serotonergic, and Purinergic Signaling