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Serine/threonine kinase Akt, an important component of the insulin signaling pathway, plays an essential role in many physiological processes. In this study, we identified and characterized an  Akt  gene (designated  LsAkt ) from the cigarette beetle,  Lasioderma serricorne .  LsAkt  contains a 1614 bp open reading frame encoding a 537 amino acid protein that possesses a conserved pleckstrin homology domain and a serine/threonine kinase domain. The expression of  LsAkt  was high in pupal stages and peaked in day-4 female pupae. In adult tissues,  LsAkt  was highly expressed in the thorax, ovary, and midgut. The expression of  LsAkt  was induced by methoprene or bovine insulin  in vivo , but significantly decreased by 20-hydroxyecdysone. RNA interference-mediated knockdown of  LsAkt  resulted in severely blocked ovarian development and reduced fecundity and hatchability. The vitellogenin (Vg) content and juvenile hormone (JH) titers of  LsAkt -depletion beetles were decreased, and expressions of  Vg  and four JH signaling and biosynthetic genes were significantly decreased. Silencing of  LsAkt  reduced the amounts of glucose, glycogen, and trehalose in female adults and affected the expressions of seven key carbohydrate metabolic genes. Taken together, it is inferred that  Akt  implicates in  L. serricorne  reproduction by modification of Vg synthesis, juvenile hormone production and carbohydrate metabolism.

Disruption of the Serine/Threonine Kinase Akt Gene Affects Ovarian Development and Fecundity in the Cigarette Beetle, Lasioderma serricorne