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Cerebral arterial vasoreactivity is vital to the regulation of cerebral blood flow. Depolarization of arterial myocytes elicits whole-cell Ca 2+  oscillations as well as subcellular Ca 2+  sparks due to activation of ryanodine receptors on the sarcoplasmic reticulum. Previous evidence illustrates that contraction of cerebral arteries from sheep and underlying Ca 2+  signaling pathways are modified by age and that long-term hypoxia (LTH) causes aberrations in Ca 2+  signaling pathways and downstream effectors impacting vasoregulation. We hypothesize that age and LTH affect the influence of membrane depolarization on whole-cell intracellular Ca 2+  oscillations and sub-cellular Ca 2+  spark activity in cerebral arteries. To test this hypothesis, we examined Ca 2+  oscillatory and spark activities using confocal fluorescence imaging techniques of Fluo-4 loaded basilar arterial myocytes of low- and high-altitude term fetal (∼145 days of gestation) and adult sheep, where high-altitude pregnant and non-pregnant sheep were placed at 3,801 m for &amp;gt;100 days. Ca 2+  oscillations and sparks were recorded using an  in situ  preparation evaluated in the absence or presence of 30 mM K +  (30K) to depolarize myocytes. Myocytes from adult animals tended to have a lower basal rate of whole-cell Ca 2+  oscillatory activity and 30K increased the activity within cells. LTH decreased the ability of myocytes to respond to depolarization independent of age. These observations illustrate that both altitude and age play a role in affecting whole-cell and localized Ca 2+  signaling, which are important to arterial vasoreactivity and cerebral blood flow.

Long-Term Hypoxia Negatively Influences Ca2+ Signaling in Basilar Arterial Myocytes of Fetal and Adult Sheep