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Myzus persicae  (Sulzer), commonly known as the green peach aphid, is a notorious pest that causes substantial losses to a range of crops and can transmit several plant viruses, including potato virus Y (PVY). Chemical insecticides provide only partial control of this pest and their use is not environmentally sustainable. In recent years, many genes related to growth, development, and reproduction have been used as targets for pest control. These include  Gustavus  ( Gus ), a highly conserved gene that has been reported to play an essential part in the genesis of germline cells and, hence, in fecundity in the model insect  Drosophila melanogaster . We hypothesized that the  Gustavus  ( Gus ) gene was a potential target that could be used to regulate the  M. persicae  population. In this study, we report the first investigation of an ortholog of  Gus  in  M. persicae , designated  MpGus , and describe its role in the fecundity of this insect. First, we identified the  MpGus  mRNA sequence in the  M. persicae  transcriptome database, verified its identity with reverse transcription-polymerase chain reaction (RT-PCR), and then evaluated the transcription levels of  MpGus  in  M. persicae  nymphs of different instars and tissues with real-time quantitative PCR (RT-qPCR). To investigate its role in regulating the fecundity of  M. persicae , we used RNA interference (RNAi) to silence the expression of  MpGus  in adult insects; this resulted in a significant reduction in the number of embryos (50.6%,  P  < 0.01) and newborn nymphs (55.7%,  P  < 0.01) in the treated aphids compared with controls. Interestingly,  MpGus  was also significantly downregulated in aphids fed on tobacco plants that had been pre-infected with PVY  N  , concomitant with a significant reduction (34.1%,  P  < 0.01) in  M. persicae  fecundity. Collectively, these data highlight the important role of  MpGus  in regulating fecundity in  M. persicae  and indicate that  MpGus  is a promising RNAi target gene for control of this pest species.

The Gustavus Gene Can Regulate the Fecundity of the Green Peach Aphid, Myzus persicae (Sulzer)