English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The ocular albinism type 1 (OA1), a pigment cell-specific integral membrane glycoprotein, is a member of the G-protein-coupled receptor (GPCR) superfamily that binds to heterotrimeric G proteins in mammalian cells. We aimed to characterize the physiological functions an insect OA1 from  Lymantria dispar  ( LdOA1 ) employs in the regulation of insecticide tolerance. In the present study, we investigated the roles of  LdOA1  in response to deltamethrin exposure in both  L. dispar  and  Drosophila melanogaster .  LdOA1  was expressed at the lowest level during the 4 th  instar stage, while  LdOA1  was significantly upregulated in the 5 th  instar and male stages. Knockdown of  LdOA1  by injecting dsRNA of  LdOA1  into gypsy moth larvae caused a 4.80-fold higher mortality than in control larvae microinjected with dsRNA of  GFP  under deltamethrin stress. Nine out of 11  L. dispar CYP  genes were significantly downregulated under deltamethrin stress in  LdOA1  silenced larvae as compared to control larvae. Moreover, the  LdOA1  gene was successfully overexpressed in  D. melanogaster  using transgenic technique. The deltamethrin contact assay showed that the  LdOA1  overexpression in flies significantly enhanced the tolerance to deltamethrin compared to the control flies. Furthermore, the downstream  Drosophila  CYP genes were upregulated in the  LdOA1  overexpression flies, suggesting  LdOA1  may play a master switch role in P450-mediated metabolic detoxification. This study is the first report of an insect OA1 gene regulating insecticide tolerance and potentially playing a role in the regulation of downstream cytochrome P450 expression. These results contribute to the future development of novel insecticides targeting insect GPCRs.

Ocular Albinism Type 1 Regulates Deltamethrin Tolerance in Lymantria dispar and Drosophila melanogaster