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MORN5 (MORN repeat containing 5) is encoded by a locus positioned on chromosome 17 in the chicken genome. The MORN motif is found in multiple copies in several proteins including junctophilins or phosphatidylinositol phosphate kinase family and the MORN proteins themselves are found across the animal and plant kingdoms. MORN5 protein has a characteristic punctate pattern in the cytoplasm in immunofluorescence imaging. Previously,  MORN5  was found among differentially expressed genes in a microarray profiling experiment of the chicken embryo head. Here, we provided  in situ  hybridization to analyse, in detail, the  MORN5  expression in chick craniofacial structures. The expression of  MORN5  was first observed at stage HH17-18 (E2.5).  MORN5  expression gradually appeared on either side of the primitive oral cavity, within the maxillary region. At stage HH20 (E3), prominent expression was localized in the mandibular prominences lateral to the midline. From stage HH20 up to HH29 (E6), there was strong expression in restricted regions of the maxillary and mandibular prominences. The frontonasal mass (in the midline of the face) expressed MORN5, starting at HH27 (E5). The expression was concentrated in the corners or globular processes, which will ultimately fuse with the cranial edges of the maxillary prominences.  MORN5  expression was maintained in the fusion zone up to stage HH29. In sections  MORN5  expression was localized preferentially in the mesenchyme. Previously, we examined signals that regulate  MORN5  expression in the face based on a previous microarray study. Here, we validated the array results with  in situ  hybridization and QPCR.  MORN5  was downregulated 24 h after Noggin and/or RA treatment. We also determined that BMP pathway genes are downstream of  MORN5  following siRNA knockdown. Based on these results, we conclude that  MORN5  is both regulated by and required for BMP signaling. The restricted expression of  MORN5  in the lip fusion zone shown here supports the human genetic data in which  MORN5  variants were associated with increased risk of non-syndromic cleft lip with or without cleft palate.

MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGFβ Pathways