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Nuclear Ca 2+  is important for the regulation of several nuclear processes such as gene expression. Localized Ca 2+  signals (LCSs) in skeletal muscle fibers of mice have been mainly studied as Ca 2+  release events from the sarcoplasmic reticulum. Their location with regard to cell nuclei has not been investigated. Our study is based on the hypothesis that LCSs associated with nuclei are present in skeletal muscle fibers of adult mice. Therefore, we carried out experiments addressing this question and we found novel Ca 2+  signals associated with nuclei of skeletal muscle fibers (with possibly attached satellite cells). We measured localized nuclear and perinuclear Ca 2+  signals (NLCSs and PLCSs) alongside cytosolic localized Ca 2+  signals (CLCSs) during a hypertonic treatment. We also observed NLCSs under isotonic conditions. The NLCSs and PLCSs are Ca 2+  signals in the range of micrometer [FWHM (full width at half maximum): 2.75 ± 0.27 μm (NLCSs) and 2.55 ± 0.17 μm (PLCSs), S.E.M.]. Additionally, global nuclear Ca 2+  signals (NGCSs) were observed. To investigate which type of Ca 2+  channels contribute to the Ca 2+  signals associated with nuclei in skeletal muscle fibers, we performed measurements with the RyR blocker dantrolene, the DHPR blocker nifedipine or the IP 3 R blocker Xestospongin C. We observed Ca 2+  signals associated with nuclei in the presence of each blocker. Nifedipine and dantrolene had an inhibitory effect on the fraction of fibers with PLCSs. The situation for the fraction of fibers with NLCSs is more complex indicating that RyR is less important for the generation of NLCSs compared to the generation of PLCSs. The fraction of fibers with NLCSs and PLCSs is not reduced in the presence of Xestospongin C. The localized perinuclear and intranuclear Ca 2+  signals may be a powerful tool for the cell to regulate adaptive processes as gene expression. The intranuclear Ca 2+  signals may be particularly interesting in this respect.

Localized nuclear and perinuclear Ca2+ signals in intact mouse skeletal muscle fibers