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The giant mudskipper,  Periophthalmodon schlosseri , is an amphibious fish that builds burrows in the mudflats. It can actively excrete ammonia through its gills, and tolerate high environmental ammonia. This study aimed to examine the effects of seawater (salinity 30; SW) acclimation and/or environmental ammonia exposure on the kinetic properties of Na + /K + -ATPase (Nka) from, and mRNA expression and protein abundance of  nka /Nka α–subunit isoforms in, the gills of  P. schlosseri  pre-acclimated to slightly brackish water (salinity 3; SBW). Our results revealed that the Nka from the gills of  P. schlosseri  pre-acclimated to SBW for 2 weeks had substantially higher affinity to (or lower  K   m   for) K +  than NH +  4 , and its affinity to NH +  4  decreased significantly after 6-days exposure to 75 mmol l −1  NH 4 Cl in SBW. Hence, Nka transported K +  selectively to maintain intracellular K +  homeostasis, instead of transporting NH +  4  from the blood into ionocytes during active NH +  4  excretion as previously suggested. Two  nka α isoforms,  nka α 1  and  nka α 3 , were cloned and sequenced from the gills of  P. schlosseri . Their deduced amino acid sequences had K +  binding sites identical to that of Nkaα1c from  Anabas testudineus , indicating that they could effectively differentiate K +  from NH +  4 . Six days of exposure to 75 mmol l −1  NH 4 Cl in SBW, or to SW with or without 50 mmol l −1  NH 4 Cl led to significant increases in Nka activities in the gills of  P. schlosseri . However, a significant increase in the comprehensive Nkaα protein abundance was observed only in the gills of fish exposed to 50 mmol l −1  NH 4 Cl in SW. Hence, post-translational modification could be an important activity modulator of branchial Nka in  P. schlosseri . The fast modulation of Nka activity and concurrent expressions of two branchial  nka α isoforms could in part contribute to the ability of  P. schlosseri  to survive abrupt transfer between SBW and SW or abrupt exposure to ammonia.

Functional roles of Na+/K+-ATPase in active ammonia excretion and seawater acclimation in the giant mudskipper, Periophthalmodon schlosseri