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NLRP3 inflammasome has been implicated in impaired post-injury muscle healing and in muscle atrophy. Histamine receptors play an important role in inflammation, but the role of histamine H 3  receptor (H 3 R) in myocyte regeneration and in the regulation of NLRP3 inflammasome is not known. We studied the effects of H 3 R signaling on C2C12 myocyte viability, apoptosis, and tumor necrosis factor alpha (TNFα)-induced NLRP3 inflammasome activation during striated myogenic differentiation at three time points (days 0, 3, and 6). Expression of  Nlrp3 , interleukin-1β (IL-1β), and myogenesis markers were determined. TNFα reduced overall viability of C2C12 cells, and exposure to TNFα induced apoptosis of cells at D6. Activation of H 3 R had no effect on viability or apoptosis, whereas inhibition of H 3 R increased TNFα-induced apoptosis. Stimulation of C2C12 cells with TNFα increased  Nlrp3  mRNA expression at D3 and D6. Moreover, TNFα reduced the expression of myogenesis markers MyoD1, Myogenin, and Myosin-2 at D3 and D6. H 3 R attenuated TNFα-induced expression of  Nlrp3  and further inhibited the myogenesis marker expression; while H 3 R -blockage enhanced the proinflammatory effects of TNFα and increased the myogenesis marker expression. TNFα-induced secretion of mature IL-1β was dependent on the activation of the NLRP3 inflammasome, as shown by the reduced secretion of mature IL-1β upon treatment of the cells with the small molecule inhibitor of the NLRP3 inflammasome (MCC950). The activation of H 3 R reduced TNFα-induced IL-1β secretion, while the H 3 R blockage had an opposite effect. In conclusion, the modulation of H 3 R activity regulates the effects of TNFα on C2C12 myocyte differentiation and TNFα-induced activation of NLRP3 inflammasome. Thus, H 3 R signaling may represent a novel target for limiting postinjury muscle inflammation and muscle atrophy.

Histamine H3 Receptor Signaling Regulates the NLRP3 Inflammasome Activation in C2C12 Myocyte During Myogenic Differentiation