English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The radioresistance of tumors affect the outcome of radiotherapy. Accumulating data suggest that 1α,25(OH) 2 D 3  is a potential anti-oncogenic molecule in various cancers. In the present study, we investigated the radiosensitive effects and underlying mechanisms of 1α,25(OH) 2 D 3   in vitro  and  in vivo . We found that 1α,25(OH) 2 D 3  enhanced the radiosensitivity of lung cancer and ovarian cancer cells by promoting the NADPH oxidase-ROS-apoptosis axis. Compared to the group that only received radiation, the survival fraction and self-renewal capacity of cancer cells treated with a combination of 1α,25(OH) 2 D 3  and radiation were decreased. Both apoptosis and ROS were significantly increased in the combination group compared with the radiation only group. Moreover, N-acetyl-L-cysteine, a scavenger of intracellular ROS, reversed the apoptosis and ROS induced by 1α,25(OH) 2 D 3 , indicating that 1α,25(OH) 2 D 3  enhanced the radiosensitivity of cancer cells  in vitro  by promoting ROS-induced apoptosis. Moreover, our results demonstrated that 1α,25(OH) 2 D 3  promoted the ROS level  via  activating NADPH oxidase complexes, NOX4, p22 phox , and p47 phox . In addition, knockdown of the vitamin D receptor (VDR) abolished the radiosensitization of 1α,25(OH) 2 D 3 , which confirmed that 1α,25(OH) 2 D 3  radiosensitized tumor cells that depend on VDR. Similarly, our study also evidenced that vitamin D 3  enhanced the radiosensitivity of cancer cells  in vivo  and extended the overall survival of mice with tumors. In summary, these results demonstrate that 1α,25(OH) 2 D 3  enhances the radiosensitivity depending on VDR and activates the NADPH oxidase-ROS-apoptosis axis. Our findings suggest that 1α,25(OH) 2 D 3  in combination with radiation enhances lung and ovarian cell radiosensitivity, potentially providing a novel combination therapeutic strategy.

1α,25(OH)2D3 Radiosensitizes Cancer Cells by Activating the NADPH/ROS Pathway