English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Background  The prevalence of cardiovascular diseases (CVDs) is increasing at a high rate, and the available treatment options, sometimes, have complications which necessitates the need to develop safer and efficacious approaches. Ethnomedicinal applications reportedly reduce CVD risk.  Ulmus parvifolia  Jacq. (Ulmaceae) commonly known as Chinese Elm or Lacebark Elm, is native to China, Japan, and Korea. It exhibits anti-inflammatory, antiviral, and anticancer properties, but its anti-platelet properties have not yet been elucidated.    Purpose  To investigate the pharmacological anti-platelet and anti-thrombotic effects of  U. parvifolia  bark extract.    Study Design and Methods  Human and rat washed platelets were prepared; light transmission aggregometry and scanning electron microscopy was performed to assess platelet aggregation and the change in platelet shape, respectively. Intracellular calcium mobilization, ATP release, and thromboxane-B2 production were also measured. Integrin α IIb β 3  activation was analyzed in terms of fibrinogen binding, fibronectin adhesion, and clot retraction. The expression of MAPK, Src, and PI3K/Akt pathway proteins was examined. Cyclic nucleotide signaling pathway was evaluated  via  cAMP elevation and VASP phosphorylation. Anti-thrombotic activity of the extract was evaluated  in vivo  using an arteriovenous shunt rat model, whereas its effect on hemostasis in mice was assessed  via  bleeding time assay.    Results    U. parvifolia  extract significantly inhibited human and rat platelet aggregation in a dose-dependent manner along with inhibition of calcium mobilization, dense granule secretion, and TxB2 production. Integrin α IIb β 3  mediated inside-out and outside-in signaling events, as evidenced by the inhibition of fibrinogen binding, fibronectin adhesion, and clot retraction. The extract significantly reduced phosphorylation of Src, MAPK (ERK, JNK, and p38 MAPK ), and PI3K/Akt pathway proteins. Cyclic-AMP levels were elevated in  U. parvifolia- treated platelets, while PKAαβγ and VASP ser157  phosphorylation was enhanced.  U. parvifolia  reduced thrombus weight in rats and moderately increased bleeding time in mice.    Conclusion    U. parvifolia  modulates platelet responses and inhibit thrombus formation by regulating integrin α IIb β 3  mediated inside-out and outside-in signaling events and cAMP signaling pathway.

Ulmus parvifolia Modulates Platelet Functions and Inhibits Thrombus Formation by Regulating Integrin αIIbβ3 and cAMP Signaling