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Mondia whitei  (Hook.f.) Skeels is an important endangered medicinal and commercial plant in South Africa.  In vitro  propagation systems are required for biomass production and bioactivity analysis to supplement wild resources/stocks. Adventitious roots from somatic embryogenic explants using suspension culture and  ex vitro -grown plants produced via somatic embryogenesis were established using different plant growth regulator treatments. The adventitious root biomass and different parts of  ex vitro -grown and mother plants were used to investigate the potential for acetylcholinesterase (AChE) and antibacterial activities. Adventitious roots derived from 2.5 μM indole-3-acetic acid (IAA) treatments and  ex vitro- grown plants derived from  meta -topolin riboside and IAA treatments gave the best AChE and antibacterial activities. The  in vitro- established  M. whitei  and  ex vitro  biomass have comparable ability to function as inhibitors of acetylcholinesterase and antibacterial agents, and can be used as potent bioresources in traditional medicine.

Acetylcholinesterase-Inhibition and Antibacterial Activity of Mondia whitei Adventitious Roots and Ex vitro-Grown Somatic Embryogenic-Biomass