English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Objectives  Macrophage-derived exosomes (Mφ-Exos) are involved in tumor onset, progression, and metastasis, but their regulation in oral squamous cell carcinoma (OSCC) is not fully understood.  RBPJ  is implicated in macrophage activation and plasticity. In this study, we assessed the role of Mφ-Exos with  RBPJ  overexpression ( RBPJ-OE  Mφ-Exos) in OSCC.    Materials and Methods  The long non-coding RNA (lncRNA) profiles in  RBPJ-OE  Mφ-Exos and THP-1-like macrophages (WT Mφ)-Exos were evaluated using lncRNA microarray. Then the functions of Mφ-Exo-lncRNA in OSCC cells were assessed  via  CCK-8, EdU, and Transwell invasion assays. Besides, luciferase reporter assay, RNA immunoprecipitation, and Pearson’s correlation analysis were adopted to confirm interactions. Ultimately, a nude mouse model of xenografts was used to further analyze the function of Mφ-Exo-lncRNAs  in vivo .    Results  It was uncovered that lncRNA  LBX1-AS1  was upregulated in  RBPJ-OE  Mφ-Exos relative to that in WT Mφ-Exos.  RBPJ-OE  Mφ-Exos and  LBX1-AS1  overexpression inhibited OSCC cells to proliferate and invade. Meanwhile,  LBX1-AS1  knockdown boosted the tumor to grow  in vivo . The effects of  RBPJ-OE  Mφ-Exos on OSCC cells can be reversed by the  LBX1-AS1  knockdown. Additionally, mechanistic investigations revealed that  LBX1-AS1  acted as a competing endogenous RNA of miR-182-5p to regulate the expression of  FOXO3 .    Conclusion  Exo- LBX1-AS1  secreted from  RBPJ-OE  Mφ inhibits tumor progression through the  LBX1-AS1 /miR-182-5p/ FOXO3  pathway, and  LBX1-AS1  is probably a diagnostic biomarker and potential target for OSCC therapy.

Exosomal LncRNA LBX1-AS1 Derived From RBPJ Overexpressed-Macrophages Inhibits Oral Squamous Cell Carcinoma Progress via miR-182-5p/FOXO3