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In this study, we isolated and identified four compounds in  Delphinium brunonianum  Royle, and they were Delbrunine ( 1 ), 4- O -α-D-Glucosyl benzoic acid ( 2 ), Kaempferol 3- O -β-D-glucopyranoside 7- O -α-L-rhamnopyranoside ( 3 ) and Eldeline ( 4 ). Furthermore, the anti-inflammatory activity of these compounds was screened in RAW264.7 cells. The results showed that the anti-inflammatory activities of compounds  2  and  3  were weak, and  1 ,  4  had good anti-inflammatory activity. The macrophage inflammation model was established by lipopolysaccharide (LPS). Then, the anti-inflammatory activity was evaluated by ELISA kits, qRT-PCR experiment and western blot experiment. And the anti-oxidative stress activity was assessed by flow cytometry. The results showed that compounds  1 ,  4  could significantly inhibit the elevation of inflammatory factors nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and also had obvious inhibitory effects on the production of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2). In addition, compounds  1  and  4  could effectively inhibit the overexpression of reactive oxygen species (ROS) in RAW264.7 cells that activated by LPS. These results indicated that compounds  1  and  4  may exert anti-inflammatory and anti-oxidative stress effects through the NF-κB signaling pathway.

Two Alkaloids From Delphinium brunonianum Royle, Their Anti-inflammatory and Anti-oxidative Stress Activity via NF-κB Signaling Pathway