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Microalgae are a source of potentially healthy and sustainable nutrients. However, the bioaccessibility of these nutrients remains uncertain. In this study, we analyzed the biomass composition of five commercial  Chlorella  and  Auxenochlorella  strains, and  Chlorella vulgaris  heterotrophically cultivated in our laboratory. Protein accounted for 65 ± 3% (w w −1 ) dry matter (DM) in all biomasses, except for the lab-grown  C. vulgaris  that contained 20% (w w −1 ) DM protein. The fatty acids content was comparable and ranged between 7 and 10% (w w −1 ) DM. Most of the biomasses had a ω6-polyunsaturated fatty acids (PUFAs)/ω3-PUFAs ratio <4, as recommended by nutritional experts. A recently published harmonized protocol for  in vitro  digestion was used to evaluate fatty acids and protein bioaccessibilities. Protein bioaccessibility ranged between 60 and 74% for commercial  Chlorella  and  Auxenochlorella  biomasses and was 43% for the lab-grown  C. vulgaris . Fatty acids bioaccessibility was <7% in commercial biomasses and 19% in the lab-grown  C. vulgaris . Taken together, the results show that microalgae are promising sources of bioaccessible protein. The limited fatty acids bioaccessibility indicates the need for alternative upstream and downstream production strategies.

Biochemical and Nutritional Evaluation of Chlorella and Auxenochlorella Biomasses Relevant for Food Application