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Neuronal identity is generated by the cell-surface expression of clustered protocadherin (Pcdh) isoforms. In mice, 58 isoforms from three gene clusters,  Pcdh α , Pcdh β, and  Pcdh γ, are differentially expressed in neurons. Since  cis -heteromeric Pcdh oligomers on the cell surface interact homophilically with that in other neurons  in trans , it has been thought that the Pcdh isoform repertoire determines the binding specificity of synapses. We previously described the cooperative functions of isoforms from all three  Pcdh  gene clusters in neuronal survival and synapse formation in the spinal cord. However, the neuronal loss and the following neonatal lethality prevented an analysis of the postnatal development and characteristics of the clustered-Pcdh-null (Δ αβγ ) neural circuits. Here, we used two methods, one to generate the chimeric mice that have transplanted Δ αβγ  neurons into mouse embryos, and the other to generate double mutant mice harboring null alleles of both the  Pcdh  gene and the proapoptotic gene  Bax  to prevent neuronal loss. First, our results showed that the surviving chimeric mice that had a high contribution of Δ αβγ  cells exhibited paralysis and died in the postnatal period. An analysis of neuronal survival in postnatally developing brain regions of chimeric mice clarified that many Δ αβγ  neurons in the forebrain were spared from apoptosis, unlike those in the reticular formation of the brainstem. Second, in Δ αβγ/Bax  null double mutants, the central pattern generator (CPG) for locomotion failed to create a left-right alternating pattern even in the absence of neurodegeneraton. Third, calcium imaging of cultured hippocampal neurons showed that the network activity of Δ αβγ  neurons tended to be more synchronized and lost the variability in the number of simultaneously active neurons observed in the control network. Lastly, a comparative analysis for  trans -homophilic interactions of the exogenously introduced single Pcdh-γA3 isoforms between the control and the Δ αβγ  neurons suggested that the isoform-specific  trans -homophilic interactions require a complete match of the expressed isoform repertoire at the contacting sites between interactive neurons. These results suggested that combinations of clustered Pcdh isoforms are required for building appropriate neural circuits.

Clustered Protocadherins Are Required for Building Functional Neural Circuits