English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Resistance acquisition  via  natural transformation is a common process in the  Neisseria  genus. Transformation has played an important role in the emergence of resistance to many antimicrobials in  Neisseria gonorrhoeae  and  Neisseria meningitidis . In a previous study, we found that currently circulating isolates of  Neisseria subflava  had acquired an  msr(D)  gene that has been found to result in macrolide resistance in other bacteria but never found in  Neisseria  species before. To determine if this resistance mechanism is transferable among  Neisseria  species, we assessed if we could transform the  msr(D)  gene into other commensal and pathogenic  Neisseria  under low dose azithromycin pressure. Intraspecies recombination in commensal  N. subflava  was confirmed with PCR and resulted in high-level macrolide resistance. Whole-genome sequencing of these transformed strains identified the complete uptake of the  msr(D)  integration fragment. Sequence analysis showed that a large fragment of DNA (5 and 12 kb) was transferred through a single horizontal gene transfer event. Furthermore, uptake of the  msr(D)  gene had no apparent fitness cost. Interspecies transformation of  msr(D)  from  N. subflava  to  N. gonorrhoeae  was, however, not successful.

Successful Intra- but Not Inter-species Recombination of msr(D) in Neisseria subflava