English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Nematicidal potential of the common plant pathogen  Pseudomonas syringae  has been recently identified against  Caenorhabditis elegans . The current study was designed to investigate the detailed genetic mechanism of the bacterial pathogenicity by applying comparative genomics, transcriptomics, mutant library screening, and protein expression. Results showed that  P. syringae  strain MB03 could kill  C. elegans  in the liquid assay by gut colonization. The genome of  P. syringae  MB03 was sequenced and comparative analysis including multi locus sequence typing, and genome-to-genome distance placed MB03 in phylogroup II of  P. syringae . Furthermore, comparative genomics of MB03 with nematicidal strains of  Pseudomonas aeruginosa  (PAO1 and PA14) predicted 115 potential virulence factors in MB03. However, genes for previously reported nematicidal metabolites, such as phenazine, pyochelin, and pyrrolnitrin, were found absent in the MB03 genome. Transcriptomics analysis showed that the growth phase of the pathogen considerably affected the expression of virulence factors, as genes for the flagellum, glutamate ABC transporter,  phoP / phoQ ,  fleS / fleR , type VI secretion system, and serralysin were highly up-regulated when stationary phase MB03 cells interacted with  C. elegans.  Additionally, screening of a transposon insertion mutant library led to the identification of other nematicidal genes such as  acnA, gltP, oprD , and  zapE . Finally, the nematicidal activity of selected proteins was confirmed by heterologous expression in  Escherichia coli .

frontiers in microbiology

Identification of the Genes of the Plant Pathogen Pseudomonas syringae MB03 Required for the Nematicidal Activity Against Caenorhabditis elegans Through an Integrated Approach