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Aflatoxins are naturally occurring high-toxic secondary metabolites, which cause worldwide environmental contaminations and wastes of food and feed resources and severely threaten human health. Thus, the highly efficient methods and technologies for detoxification of aflatoxins are urgently needed in a long term. In this work, we report the construction of recombinant  Kluyveromyces lactis  strains GG799(pKLAC1-Phs mnp ), GG799(pKLAC1-Plo mnp ), GG799(pKLAC1-Phc mnp ), and then the food-grade expression of the three manganese peroxidases in these strains, followed by the degradation of aflatoxin B 1  (AFB 1 ) using the fermentation supernatants. The expression of the manganese peroxidases was achieved in a food-grade manner since  Kluyveromyces lactis  is food-safe and suitable for application in food or feed industries. The inducible expression process of the optimal recombinant strain GG799(pKLAC1-Phc mnp ) and the aflatoxin B 1  degradation process were both optimized in detail. After optimization, the degradation ratio reached 75.71%, which was an increase of 49.86% compared to the unoptimized results. The degradation product was analyzed and determined to be AFB 1 -8,9-dihydrodiol. The recombinant strain GG799(pKLAC1-Phc mnp ) supernatants degraded more than 90% of AFB 1  in the peanut samples after twice treatments. The structural computational analysis for further mutagenesis of the enzyme PhcMnp was also conducted in this work. The food-grade recombinant yeast strain and the enzyme PhcMnp have potential to be applied in food or feed industries.

Food-Grade Expression of Manganese Peroxidases in Recombinant Kluyveromyces lactis and Degradation of Aflatoxin B1 Using Fermentation Supernatants