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Our current knowledge on bacterial cadmium (Cd) resistance is mainly based on the functional exploration of specific Cd-resistance genes. In this study, we carried out a genomic study on Cd resistance of a newly isolated  Cellulomonas  strain with a MIC of 5 mM Cd. Full genome of the strain, with a genome size of 4.47 M bp and GC-content of 75.35%, was obtained through high-quality sequencing. Genome-wide annotations identified 54 heavy metal-related genes. Four potential Cd-resistance genes, namely  zntAY8 ,  copAY8 ,  HMTY8 , and  czcDY8 , were subjected to functional exploration. Quantitative PCR determination of  in vivo  expression showed that  zntAY8, copAY8 , and  HMTY8  were strongly Cd-inducible. Expression of the three inducible genes against time and Cd concentrations were further quantified. It is found that  zntAY8  responded more strongly to higher Cd concentrations, while expression of  copAY8  and  HMTY8  increased over time at lower Cd concentrations. Heterologous expression of the four genes in Cd-sensitive  Escherichia coli  led to different impacts on hosts’ Cd sorption, with an 87% reduction by  zntAY8  and a 3.7-fold increase by  HMTY8 . In conclusion, a Cd-resistant  Cellulomonas  sp. strain was isolated, whose genome harbors a diverse panel of metal-resistance genes. Cd resistance in the strain is not controlled by a dedicated gene alone, but by several gene systems collectively whose roles are probably time- and dose-dependent. The plasmid-free, high-GC strain Y8 may provide a platform for exploring heavy metal genomics of the  Cellulomonas  genus.

Genomic Insights Into Cadmium Resistance of a Newly Isolated, Plasmid-Free Cellulomonas sp. Strain Y8