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Bacterial fruit blotch, caused by seed-borne pathogen  Acidovorax citrulli , poses a serious threat to the production of cucurbits globally. Although the disease can cause substantial economic losses, limited information is available about the molecular mechanisms of virulence. This study identified that, a random transposon insertion mutant impaired in the ability to elicit a hypersensitive response on tobacco. The disrupted gene in this mutant was determined to be  Aave_0638 , which is predicted to encode a YggS family pyridoxal phosphate-dependent enzyme. YggS is a highly conserved protein among multiple organisms, and is responsible for maintaining the homeostasis of pyridoxal 5′-phosphate and amino acids in cells.  yggS  deletion mutant of  A. citrulli  strain XjL12 displayed attenuated virulence, delayed hypersensitive response, less tolerance to H 2 O 2  and pyridoxine, increased sensitivity to antibiotic β-chloro-D-alanine, and reduced swimming. In addition, RNA-Seq analysis demonstrated that  yggS  was involved in regulating the expression of certain pathogenicity-associated genes related to secretion, motility, quorum sensing and oxidative stress response. Importantly, YggS significantly affected type III secretion system and its effectors  in vitro . Collectively, our results suggest that YggS is indispensable for  A.citrulli  virulence and expands the role of YggS in the biological processes.

yggS Encoding Pyridoxal 5′-Phosphate Binding Protein Is Required for Acidovorax citrulli Virulence