English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Toxoplasma gondii  is an obligate intracellular protozoan parasite, which has a worldwide distribution and can infect a large number of warm-blooded animals and humans.  T. gondii  must colonize and proliferate inside the host cells in order to maintain its own survival by securing essential nutrients for the development of the newly generated tachyzoites. The type II fatty acid biosynthesis pathway (FASII) in the apicoplast is essential for the growth and survival of  T. gondii . We investigated whether deletion of genes in the FASII pathway influences the  in vitro  growth and  in vivo  virulence of  T. gondii . We focused on beta-hydroxyacyl-acyl carrier protein dehydratase (FabZ) and oxidoreductase, short chain dehydrogenase/reductase family proteins ODSCI and ODSCII. We constructed  T. gondii  strains deficient in  FabZ, ODSCI , and  ODSCII  using CRISPR-Cas9 gene editing technology. The results of immunofluorescence assay, plaque assay, proliferation assay and egress assay showed that in RHΔ FabZ  strain the apicoplast was partly lost and the growth ability of the parasite  in vitro  was significantly inhibited, while for RHΔ ODSCI  and RHΔ ODSCII  mutant strains no similar changes were detected. RHΔ FabZ  exhibited reduced virulence for mice compared with RHΔ ODSCI  and RHΔ ODSCII , as shown by the improved survival rate. Deletion of  FabZ  in the PRU strain significantly decreased the brain cyst burden in mice compared with PRUΔ ODSCI  and PRUΔ ODSCII . Collectively, these findings suggest that FabZ contributes to the growth and virulence of  T. gondii , while ODSCI and ODSCII do not contribute to these traits.

The Role of Type II Fatty Acid Synthesis Enzymes FabZ, ODSCI, and ODSCII in the Pathogenesis of Toxoplasma gondii Infection