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The enhancement of nitrogen fixation activity of diazotrophs is essential for safe crop production. Lysine succinylation (K Suc ) is widely present in eukaryotes and prokaryotes and regulates various biological process. However, knowledge of the extent of K Suc  in nitrogen fixation of  Azotobacter chroococcum  is scarce. In this study, we found that 250 mg/l of rhamnolipid (RL) significantly increased the nitrogen fixation activity of  A. chroococcum  by 39%, as compared with the control. Real-time quantitative reverse transcription PCR (qRT-PCR) confirmed that RL could remarkably increase the transcript levels of  nifA  and  nifHDK  genes. In addition, a global K Suc  of  A. chroococcum  was profiled using a 4D label-free quantitative proteomic approach. In total, 5,008 K Suc  sites were identified on 1,376 succinylated proteins. Bioinformatics analysis showed that the addition of RL influence on the K Suc  level, and the succinylated proteins were involved in various metabolic processes, particularly enriched in oxidative phosphorylation, tricarboxylic acid cycle (TCA) cycle, and nitrogen metabolism. Meanwhile, multiple succinylation sites on MoFe protein (NifDK) may influence nitrogenase activity. These results would provide an experimental basis for the regulation of biological nitrogen fixation with K Suc  and shed new light on the mechanistic study of nitrogen fixation.

Rhamnolipid Enhances the Nitrogen Fixation Activity of Azotobacter chroococcum by Influencing Lysine Succinylation