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Woodchip bioreactors are increasingly used to remove nitrate (NO 3  – ) from agricultural drainage water in order to protect aquatic ecosystems from excess nitrogen. Nitrate removal in woodchip bioreactors is based on microbial processes, but the microbiomes and their role in bioreactor efficiency are generally poorly characterized. Using metagenomic analyses, we characterized the microbiomes from 3 full-scale bioreactors in Denmark, which had been operating for 4–7 years. The microbiomes were dominated by  Proteobacteria  and especially the genus  Pseudomonas , which is consistent with heterotrophic denitrification as the main pathway of NO 3  –  reduction. This was supported by functional gene analyses, showing the presence of the full suite of denitrification genes from NO 3  –  reductases to nitrous oxide reductases. Genes encoding for dissimilatory NO 3  –  reduction to ammonium were found only in minor proportions. In addition to NO 3  –  reducers, the bioreactors harbored distinct functional groups, such as lignocellulose degrading fungi and bacteria, dissimilatory sulfate reducers and methanogens. Further, all bioreactors harbored genera of heterotrophic iron reducers and anaerobic iron oxidizers ( Acidovorax ) indicating a potential for iron-mediated denitrification. Ecological indices of species diversity showed high similarity between the bioreactors and between the different positions along the flow path, indicating that the woodchip resource niche was important in shaping the microbiome. This trait may be favorable for the development of common microbiological strategies to increase the NO 3  –  removal from agricultural drainage water.

Microbiome Structure and Function in Woodchip Bioreactors for Nitrate Removal in Agricultural Drainage Water