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Patched (Ptc) and Patched-related (Ptr) proteins containing sterol-sensing domains (SSD) and Patched domains are highly conserved in eukaryotes for lipid transport and metabolism. Four proteins containing predicted SSD and Patched domains were simultaneously found by searching the  Phytophthora sojae  genome database, and one of them was identified as a Patched-like (PTL) protein. Here, we investigated the biological function of  PsPTL . The expression level of  PsPTL  was higher during mycelial and sporulation stages, compared to zoospore (ZO), cyst, and germinated-cyst stages, without significant change during infection. However, deletion of  PsPTL  using CRISPR/Cas9 had no significant effect on the growth, development, or virulence of  P. sojae . Further investigations showed that  PsPTL  is not essential for  P. sojae  to cope with external stresses such as temperature, pH, oxidative and osmotic pressure. In addition, this gene did not appear to play an essential role in  P. sojae ’s response to exogenous sterols. The transcript levels of the other three proteins containing predicted SSD and Patched domains were also not significantly upregulated in  PsPTL  deletion transformants. Our studies demonstrated that PsPTL is not an essential protein for  P. sojae  under the tested conditions, and more in-depth research is required for revealing the potential functions of  PsPTL  under special conditions or in other signaling pathways.

A Patched-Like Protein PsPTL Is Not Essential for the Growth and Response to Various Stresses in Phytophthora sojae