English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Mitogen-activated protein kinase (MAPK) pathways play a vital role in multiple plant processes, including growth, development, and stress signaling, but their involvement in response to  Ralstonia solanacearum  is poorly understood, particularly in pepper plants. Herein,  CaMAPK7  was identified from the pepper genome and functionally analyzed. The accumulations of  CaMAPK7  transcripts and promoter activities were both significantly induced in response to  R. solanacearum  strain FJC100301 infection, and exogenously applied phytohormones, including methyl jasmonate (MeJA), brassinolide (BR), salicylic acid (SA), and ethephon (ETN), were decreased by abscisic acid (ABA) treatment. Virus-induced gene silencing (VIGS) of  CaMAPK7  significantly enhanced the susceptibility of pepper plants to infection by  R. solanacearum  and downregulated the defense-related marker genes, including  CaDEF1 ,  CaPO2 ,  CaSAR82A , and  CaWRKY40 . In contrast, the ectopic overexpression of  CaMAPK7  in transgenic tobacco enhanced resistance to  R. solanacearum  and upregulated the defense-associated marker genes, including  NtHSR201 ,  NtHSR203 ,  NtPR4 ,  PR1a/c ,  NtPR1b ,  NtCAT1 , and  NtACC . Furthermore, transient overexpression of  CaMAPK7  in pepper leaves triggered intensive hypersensitive response (HR)-like cell death, H 2 O 2  accumulation, and enriched CaWRKY40 at the promoters of its target genes and drove their transcript accumulations, including  CaDEF1 ,  CaPO2 , and  CaSAR82A . Taken together, these data indicate that  R. solanacearum  infection induced the expression of  CaMAPK7 , which indirectly modifies the binding of CaWRKY40 to its downstream targets, including  CaDEF1 ,  CaPO2 , and  CaSAR82A , ultimately leading to the activation of pepper immunity against  R. solanacearum . The protein that responds to CaMAPK7 in pepper plants should be isolated in the future to build a signaling bridge between CaMAPK7 and CaWRKY40.

The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Ralstonia solanacearum Infection