English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Shewanella  species are widely distributed in the aquatic environment and aquatic organisms. They are opportunistic human pathogens with increasing clinical infections reported in recent years. However, there is a lack of a rapid and accurate method to identify  Shewanella  species. We evaluated here matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid identification of  Shewanella.  A peptide mass reference spectra (PMRS) database was constructed for the type strains of 36  Shewanella  species. The main spectrum projection (MSP) cluster dendrogram showed that the type strains of  Shewanella  species can be effectively distinguished according to the different MS fingerprinting. The PMRS database was validated using 125  Shewanella  test strains isolated from various sources and periods; 92.8% ( n  = 116) of the strains were correctly identified at the species level, compared with the results of multilocus sequence analysis (MLSA), which was previously shown to be a method for identifying  Shewanella  at the species level. The misidentified strains ( n  = 9) by MALDI-TOF MS involved five species of two groups, i.e.,  Shewanella algae – Shewanella chilikensis – Shewanella indica  and  Shewanella seohaensis – Shewanella xiamenensis . We then identified and defined species-specific biomarker peaks of the 36 species using the type strains and validated these selected biomarkers using 125 test strains. Our study demonstrated that MALDI-TOF MS was a reliable and powerful tool for the rapid identification of  Shewanella  strains at the species level.

Establishment and Application of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Detection of Shewanella Genus