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The brucellae are facultative intracellular bacteria with a cell envelope rich in phosphatidylcholine (PC). PC is abundant in eukaryotes but rare in prokaryotes, and it has been proposed that  Brucella  uses PC to mimic eukaryotic-like features and avoid innate immune responses in the host. Two PC synthesis pathways are known in prokaryotes: the PmtA-catalyzed trimethylation of phosphatidylethanolamine and the direct linkage of choline to CDP-diacylglycerol catalyzed by the PC synthase Pcs. Previous studies have reported that  B. abortus  and  B. melitensis  possess non-functional PmtAs and that PC is synthesized exclusively  via  Pcs in these strains. A putative choline transporter ChoXWV has also been linked to PC synthesis in  B. abortus . Here, we report that Pcs and Pmt pathways are active in  B. suis  biovar 2 and that a bioinformatics analysis of  Brucella  genomes suggests that PmtA is only inactivated in  B. abortus  and  B. melitensis  strains. We also show that ChoXWV is active in  B. suis  biovar 2 and conserved in all brucellae except  B. canis  and  B. inopinata.  Unexpectedly, the experimentally verified ChoXWV dysfunction in  B. canis  did not abrogate PC synthesis in a PmtA-deficient mutant, which suggests the presence of an unknown mechanism for obtaining choline for the Pcs pathway in  Brucella . We also found that ChoXWV dysfunction did not cause attenuation in  B. suis  biovar 2. The results of these studies are discussed with respect to the proposed role of PC in  Brucella  virulence and how differential use of the Pmt and Pcs pathways may influence the interactions of these bacteria with their mammalian hosts.

frontiers in microbiology

The Phospholipid N-Methyltransferase and Phosphatidylcholine Synthase Pathways and the ChoXWV Choline Uptake System Involved in Phosphatidylcholine Synthesis Are Widely Conserved in Most, but Not All Brucella Species