English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Pterocarpus angolensis , a leguminous tree native to the dry woodlands of Southern Africa, provides valuable timber, but is threatened by land conversion and overharvesting while showing limited natural regeneration. Nitrogen-fixing root nodule symbionts that could improve establishment of young seedlings have not yet been described. Therefore, we investigated the ability of  P. angolensis  to form nodules with a diverse range of rhizobia. In drought-prone areas under climate change with higher temperatures, inoculants that are heat-tolerant and adapted to these conditions are likely to be of advantage. Sources of bacterial isolates were roots of  P. angolensis  from nurseries in the Kavango region, other shrubs from this area growing near  Pterocarpus  such as  Indigofera rautanenii ,  Desmodium barbatum ,  Chamaecrista  sp., or shrubs from drought-prone areas in Namaqualand ( Wiborgia monoptera ,  Leobordea digitata ) or Kalahari ( Indigofera alternans ). Only slight protrusions were observed on  P. angolensis  roots, from which a non-nodulating  Microbacterium  sp. was isolated. Rhizobia that were isolated from nodules of other shrubs were affiliated to  Bradyrhizobium ripae  WR4 T ,  Bradyrhizobium  spp. (WR23/WR74/WR93/WR96), or  Ensifer/Mesorhizobium  (WR41/WR52). As many plant growth-promoting rhizobacteria (PGPR), nodule isolates produced siderophores and solubilized phosphate. Among them, only the  Bradyrhizobium  strains nodulated  P. angolensis  under controlled conditions in the laboratory. Isolates were further characterized by multilocus sequence analysis and were found to be distant from known  Bradyrhizobium  species. Among additional reference species tested for nodulation on  P. angolensis ,  Bradyrhizobium vignae  7-2 T  and  Bradyrhizobium namibiense  5-10 T  from the Kavango region of Namibia as well as  Bradyrhizobium elkanii  LMG6234 T  and  Bradyrhizobium yuanmingense  LMG21728 T  induced nitrogen-fixing nodules, while  Bradyrhizobium diazoefficiens  USDA110 T  and  Bradyrhizobium tropiciagri  SEMIA6148 T  did not. This suggests a broad microsymbiont range from  Bradyrhizobium japonicum  and  B. elkanii  lineages. Phylogenetic analysis of  nodC  genes indicated that nodulating bradyrhizobia did not belong to a specific symbiovar. Also, for  I. rautanenii  and  Wiborgia , nodule isolates  B. ripae  WR4 T  or  Mesorhizobium  sp. WR52, respectively, were authenticated. Characterization of symbionts inducing effective root nodules in  P. angolensis  and other shrubs from Subsahara Africa (SSA) give insights in their symbiotic partners for the first time and might help in future to develop bioinoculants for young seedlings in nurseries, and for reforestation efforts in Southern Africa.

frontiers in microbiology

Root Nodule Rhizobia From Undomesticated Shrubs of the Dry Woodlands of Southern Africa Can Nodulate Angolan Teak Pterocarpus angolensis, an Important Source of Timber