English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The ParABS partitioning system, a main driver of DNA segregation in bacteria, employs two proteins, ParA and ParB, for plasmid partition. The pMF1 plasmid from  Myxococcus fulvus  124B02 has a  par  operon encoding a small acidic protein, ParC, in addition to type I ParA and ParB homologs. Here, we show that expression of  parC  upstream of  parA  (as in the natural case), but not ectopic expression, is essential for the plasmid inheritance in  Myxococcus  cells. Co-expression of  parC  upstream of  parA  was determined to form a soluble ParC–ParA heterodimer at a 1:1 ratio, while individual expression of  parA  or co-expression of  parA  with ectopic  parC  formed insoluble ParA proteins. Purified ParA proteins alone had no ATPase activity and was easily dimerized, while mixing ParA with ParC formed the ParC–ParA heterodimer with the ATPase and polymerization activities. Fusing ParC and ParA also produced soluble proteins and some chimeras restored the ATPase activity and plasmid inheritance. The results highlight that proximal location of  parC  before  parA  is critical to realize the functions of ParA in the partition of  Myxococcus  plasmid pMF1 and shed light on a new mechanism to realize a protein function by two separate proteins.

ParC, a New Partitioning Protein, Is Necessary for the Active Form of ParA From Myxococcus pMF1 Plasmid