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Superoxide dismutase (SOD) plays an essential role in eliminating oxidative damage of lactic acid bacteria.  Streptococcus thermophilus , an important probiotic lactic acid bacterium, often inevitably suffers from various oxidative stress during dairy fermentation. In this study, to confer high-level oxidative resistance, the  sod  gene from  Lactobacillus casei  was heterologous expressed in  S. thermophilus  S-3 using our previous constructed native constitutive promoter library. The enzyme activity of SOD was significantly enhanced in engineered  S. thermophilus  by promoter #14 (2070 U/mg). Furthermore, the strategy of multi-copy  sod -expressing cassettes was employed to improve SOD activity. The maximum activity (2750 U/mg) was obtained by the two-copy  sod  recombinant, which was 1.5-fold higher than that of one-copy recombinant. In addition, the survival rate of multi-copy  sod  recombinants was increased about 97-fold with 3.5 mmol/L H 2 O 2  treatment. To our knowledge, this is the first report of multi-copy  sod  gene expression in  S. thermophilus , which exerts a positive effect on coping with oxidative stress to enhance the potential of industrial application.

Enhanced Antioxidant Activity in Streptococcus thermophilus by High-Level Expression of Superoxide Dismutase