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In this study, the transcriptional profiling of  Penicillium digitatum  after C 12 O 3 TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in  P. digitatum  were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KEGG pathways. The expression patterns of differentially expressed genes (DEGs) at 2 and 12 h were similar, mainly were the metabolic processes in cell wall, cell membrane, genetic information and energy. Particularly, the main metabolic process which was affected by C 12 O 3 TR stress for 2 and 12 h was cell integrity, including cell wall and cell membrane. The changes of chitin in cell wall was observed by Calcofluor White (CFW) staining assay. The weaker blue fluorescence in the cell wall septa, the decrease of β-1, 3-glucan synthase activity and the increase of chitinase and AKP activity showed that C 12 O 3 TR could damage the cell wall integrity. In conclusion, these results suggested that C 12 O 3 TR could inhibit the growth of  P. digitatum  through various mechanisms at transcriptional level, and could influence the cell wall permeability and integrity.

Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum