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Ferric and ferrous iron is an essential transition metal for growth of many bacterial species including mycobacteria. The genomic region  msmeg_0234  to  msmeg_0252  from  Mycobacterium smegmatis  is putatively involved in iron/heme metabolism. We investigate the genes encoding the presumed two component system MSMEG_0244/MSMEG_0246, the neighboring gene  msmeg_0243  and their involvement in this process. We show that purified MSMEG_0243 indeed is a heme binding protein. Deletion of  msmeg_0243 / msmeg_0244/msmeg_0246  in  Mycobacterium smegmatis  leads to a defect in biofilm formation and colony growth on solid agar, however, this phenotype is independent of the supplied iron source. Further, analysis of the corresponding mutant and its lipids reveals that changes in morphology and biofilm formation correlate with altered acylation patterns of phosphatidylinositol mannosides (PIMs). We provide the first evidence that  msmeg_0244/msmeg_0246  work in concert in cellular lipid homeostasis, especially in the maintenance of PIMs, with the heme-binding protein MSMEG_0243 as potential partner.

frontiers in microbiology

The Two-Component Locus MSMEG_0244/0246 Together With MSMEG_0243 Affects Biofilm Assembly in M. smegmatis Correlating With Changes in Phosphatidylinositol Mannosides Acylation