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Giardia duodenalis  is an important zoonotic parasite that can cause human and animal diarrhea.  Giardiavirus  (GLV) is a double-stranded RNA virus in Totiviridae family, which specifically infects trophozoites of the primitive protozoan parasite  G. duodenalis.  However, the GLV infectious and the pathogenicity of the  G. duodenalis  still remain to be confirmed. The GLV genome is 6,277 bp, which encodes two proteins (Gag and Gag-Pol). The expression of Gag-Pol protein is regulated by a-1 ribosomal frameshift. In this report, we identified a novel microRNA (GLV miRNA1) from the GLV. Split ligation northern results showed that GLV miRNA1 is a special expression product of GLV, and the precursor was also identified by primer extension. Antisense sequence of the GLV miRNA1 could increase the copy number of virus in  G. duodenalis . It suggests that GLV miRNA1 governs the copy number of  Giardiavirus  in  G. duodenalis . Most importantly, the GLV miRNA1 lies at the translated region of the  rdrp  gene, which is the first case that microRNA locates in the translated region of a known protein. It may be implying a novel phenomenon for miRNA biogenesis.

A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis