English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Resistance against antimicrobial peptides (AMPs) is often mediated by detoxification modules that rely on sensing the AMP through a BceAB-like ATP-binding cassette (ABC) transporter that subsequently activates a cognate two-component system (TCS) to mount the cell response. Here, the  Lactococcus lactis  ABC transporter YsaDCB is shown to constitute, together with TCS-G, a detoxification module that protects  L. lactis  against bacitracin and the bacteriocin Lcn972, both AMPs that inhibit cell wall biosynthesis. Initially, increased expression of  ysaDCB  was detected by RT-qPCR in three  L. lactis  resistant to Lcn972, two of which were also resistant to bacitracin. These mutants shared, among others, single-point mutations in  ysaB  coding for the putative Bce-like permease. These results led us to investigate the function of YsaDCB ABC-transporter and study the impact of these mutations. Expression  in trans  of  ysaDCB  in  L. lactis  NZ9000, a strain that lacks a functional detoxification module, enhanced resistance to both AMPs, demonstrating its role as a resistance factor in  L. lactis . When the three different  ysaB  alleles from the mutants were expressed, all of them outperformed the wild-type transporter in resistance against Lcn972 but not against bacitracin, suggesting a distinct mode of protection against each AMP. Moreover, P  ysaD   promoter fusions, designed to measure the activation of the detoxification module, revealed that the  ysaB  mutations unlock transcriptional control by TCS-G, resulting in constitutive expression of the  ysaDCB  operon. Finally, deletion of  ysaD  was also performed to get an insight into the function of this gene.  ysaD  encodes a secreted peptide and is part of the  ysaDCB  operon. YsaD appears to modulate signal relay between the ABC transporter and TCS-G, based on the different response of the P  ysaD   promoter fusions when it is not present. Altogether, the results underscore the unique features of this lactococcal detoxification module that warrant further research to advance in our overall understanding of these important resistance factors in bacteria.

Mutations Selected After Exposure to Bacteriocin Lcn972 Activate a Bce-Like Bacitracin Resistance Module in Lactococcus lactis