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The thermophilic archaeon  Sulfolobus acidocaldarius  can use different carbon sources for growth, including the pentoses D-xylose and L-arabinose. In this study, we identified the activator XylR ( saci_2116 ) responsible for the transcriptional regulation of the pentose transporter and pentose metabolizing genes in  S. acidocaldarius . A  xylR  deletion mutant showed growth retardation on D-xylose/L-arabinose containing media and the lack of transcription of the respective ABC transporter. In contrast to so far used promoters for expression in  S. acidocaldarius , the xylR responsive promoters have a very low background activity. Finally, two XylR dependent promoters next to the long-established maltose inducible promotor were used to construct a high-throughput expression vector system for  S. acidocaldarius  to efficiently clone and express proteins in  S. acidocaldarius .

frontiers in microbiology

Identification of XylR, the Activator of Arabinose/Xylose Inducible Regulon in Sulfolobus acidocaldarius and Its Application for Homologous Protein Expression