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Actinobacillus pleuropneumoniae  is the pathogen of porcine contagious pleuropneumonia. In  A. pleuropneumoniae , the CpxAR two-component system is essential for fitness and growth. The O-antigen protrudes from the outer membrane to the exterior of the cell, and the outer membrane serves as a barrier that helps the bacteria to survive in harsh environments. WecA, a undecaprenyl phosphate GlcNAc-1-phosphate transferase, is involved in O-antigen repeating unit biosynthesis. In this study, we investigated the role of CpxAR in the expression of  wecA  in  A. pleuropneumoniae . Our results revealed that CpxR positively regulates  wecA  expression by directly binding to the putative promoter region of  wecA . Wild-type, Δ cpxAR , Δ wecA , and complemented strains were investigated under serum, oxidative, and osmotic stresses. The Δ cpxAR  and Δ wecA  strains were more susceptible to these stresses than the wild-type, but the complemented strains showed phenotypes similar to those of the wild-type. Mice infected with the Δ cpxAR  and Δ wecA  strains exhibited lower mortality and bacterial loads in the lung than those infected with the wild-type or complemented strains. This study reveals that the CpxAR two-component system contributes to  A. pleuropneumoniae  growth, stress resistance, and virulence, by upregulating expression of  wecA.  Our findings provide new insight into the pathogenesis of  A. pleuropneumoniae .

frontiers in microbiology

The CpxAR Two-Component System Contributes to Growth, Stress Resistance, and Virulence of Actinobacillus pleuropneumoniae by Upregulating wecA Transcription